Past event
School of Chemistry Colloquium: Dr Andreas Meyer (University of Gottingen) Five years of 19F ENDOR at various field strengths: Applications in structural biology and recent developments
About five years ago, we have introduced 19F electron nuclear double resonance (ENDOR) as a spectroscopic technique to measure biomolecular distances on the order of 0.7 — 1.5 nm in paramagnetic systems at 3.4 T. Since then, we and others have presented various methodical or application-oriented studies of this spectroscopic technique.
The first part of this talk will give a summary of the methodological developments achieved at our institute. Most importantly, in our most recent studies we have worked at lower fields of 1.2 T, which is a significantly less demanding experimental set up and offers many opportunities to improve the experimental performance, such as an increased distance range or the ability to perform ENDOR experiments at much higher RF powers.
In the second part the focus will shift to biostructural applications, such as studying the radical transfer pathway in ribonucleotide reductase or the investigation of nucleic acids including a fluoride binding riboswitch.
This talk is open to final year undergraduate project students, MSc students, PhD students, post-doctoral researchers and academic staff.